A Cross Sectional Study of Trichinella in Pigs in CDR, Nepal Using Pepsin Digestion and ELISA Serology
نویسندگان
چکیده
Introduction Roundworms of the genus Trichinella are spread worldwide and are responsible for one of the most serious helminthic zoonoses. It is recognized that in many parts of the world none of the existing methods of control for Trichinella infection are applied because of the economic problems, erosions of veterinary infrastructures, failure of educational systems, ineffective abattoir control measures and non-awareness of the disease by medicals. These factors are responsible for Trichinella infection still in the food chain in large parts of the world (1). In Nepal there is a serological evidence of trichinellosis in pigs (2). In pigs high antibody titers against Trichinella reveal a recent infection because several months after the infection the antibody level begins to decreases. Therefore doubtful titers are not predictive enough in older animals. So a verification of the realistic prevalence is required using the Pepsin digestion. Objectives of this current study were; to investigate the Trichinella larval status in meat samples of slaughtered pigs by the Pepsin digestion method; to compare the results of the Pepsin digestion with corresponding serum samples by indirect antibody ELISA and finally to characterize the species of Trichinella larvae.
منابع مشابه
Cross-sectional study of Trichinella spp in pigs in CDR, Nepal using pepsin digestion and ELISA serology.
This epidemiological cross-sectional study was conducted in five major pig-producing districts of CDR, Nepal from November 2006 to April 2007. A total of 576 slaughtered pigs were randomly selected and diaphragmatic crus muscles (n = 551), corresponding sera (n = 487) and sera only (n = 25) were collected from them. Meat samples were examined by pepsin digestion to evaluate for larvae of Trichi...
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